In Business Since 2004
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Pre-B Cell Receptor Acts as a Selectivity Switch for Galectin-1 at the Pre-B Cell Surface
Summary – Galectins are glycan-binding proteins translating the sugar-encoded information of cellular glycoconjugates into physiological activities, including immunity, cell migration, and signaling. Galectins also interact with non-glycosylated partners in the extracellular milieu, among which the pre-B cell receptor (pre-BCR) during B cell development. Here, we perform NMR experiments on native membranes to monitor Gal-1 binding to physiological cell surface ligands. We show that pre-BCR interaction changes Gal-1 binding to glycosylated pre-B cell surface receptors. At the molecular and cellular levels, we identify α-2,3-sialylated motifs as key targeted epitopes. This targeting occurs through a selectivity switch increasing Gal-1 contacts with α-2,3-sialylated poly-N-acetyllactosamine upon pre-BCR interaction. Importantly, we observe that this switch is involved in the regulation of pre-BCR activation. Altogether, this study demonstrates that interactions to non-glycosylated proteins regulate the glycan-decoding functions of galectins at the cell surface. [Cell Reports 2024, 48(8), 114541.]
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Industry-Trusted Biotechnology Company and CRO
Advance your biomedical research industry with help from CASSIA LLC. Founded in 2004, we are a privately held biotechnology company and clinical research organization (CRO) specializing in green chemo-enzymatic synthesis solutions for preparing stable isotope-labeled (D, C-13, N-15, O-17/18) metabolites, proteins, nucleic acids, and their analogs.
Leveraging our unique core technology platforms and expertise, we bring responsible, cost-effective, and creative solutions to a variety of basic, translational, and biomedical research applications, such as:
- MS
- NMR
- Assay Development
- Metabolic Research
Our Facility
CASSIA LLC laboratories are located in an eight-building, 150,700 sq. ft. research and development (R&D) business park in the Mira Mesa region of San Diego, CA. We occupy 1580 sq. ft. and are capable of carrying out both basic and applied research in:
- Molecular Biology
- Biocatalysis
- Organic Synthesis
- Protein and Nucleic Acid Production
Contact us today to learn more about our biotechnology company and the products and services we provide.
Industry-Trusted Biotechnology Company and CRO
Advance your biomedical research industry with help from CASSIA LLC. Founded in 2004, we are a privately held biotechnology company and clinical research organization (CRO) specializing in green chemo-enzymatic synthesis solutions for preparing stable isotope-labeled (D, C-13, N-15, O-17/18) metabolites, proteins, nucleic acids, and their analogs.
Leveraging our unique core technology platforms and expertise, we bring responsible, cost-effective, and creative solutions to a variety of basic, translational, and biomedical research applications, such as:
- MS
- NMR
- Assay Development
- Metabolic Research
Our Facility
CASSIA LLC laboratories are located in an eight-building, 150,700 sq. ft. research and development (R&D) business park in the Mira Mesa region of San Diego, CA. We occupy 1580 sq. ft. and are capable of carrying out both basic and applied research in:
- Molecular Biology
- Biocatalysis
- Organic Synthesis
- Protein and Nucleic Acid Production
Contact us today to learn more about our biotechnology company and the products and services we provide.
Email us to learn more about our services.