In Business Since 2004

Over 18 Years of Industry Experience

Specialized Green Chemo-Enzymatic Synthesis Solutions

Member of the California Life Sciences and Biocom

Find Out More
A Diagram of a Computer System

Explore our applications

Group of Students Working at the Laboratory

Industry-Trusted Biotechnology Company and CRO

Advance your biomedical research industry with help from CASSIA LLC. Founded in 2004, we are a privately held biotechnology company and clinical research organization (CRO) specializing in green chemo-enzymatic synthesis solutions for preparing stable isotope-labeled (D, C-13, N-15, O-17/18) metabolites, proteins, nucleic acids, and their analogs. 

Leveraging our unique core technology platforms and expertise, we bring responsible, cost-effective, and creative solutions to a variety of basic, translational, and biomedical research applications, such as: 

  • MS 
  • NMR 
  • Assay Development 
  • Metabolic Research 
Interior of Clean Modern White Medical or Chemical Laboratory Background

Our Facility

CASSIA LLC laboratories are located in an eight-building, 150,700 sq. ft. research and development (R&D) business park in the Mira Mesa region of San Diego, CA. We occupy 1580 sq. ft. and are capable of carrying out both basic and applied research in: 

  • Molecular Biology 
  • Biocatalysis 
  • Organic Synthesis 
  • Protein and Nucleic Acid Production 

Contact us today to learn more about our biotechnology company and the products and services we provide. 

Explore our Latest Application Using Stable Isotope Labeled Nucleotides

 Mechanism of Rate Acceleration of Radical C-C Bond Formation Reaction by a Radical SAM GTP 3′,8-Cyclase

Summary – While the number of characterized radical S-adenosyl-L-methionine (SAM) enzymes is increasing, the roles of these enzymes in radical catalysis remain largely ambiguous. In radical SAM enzymes, the slow radical initiation step kinetically masks the subsequent steps, making it impossible to study the kinetics of radical chemistry. Here, we report the first evidence that a radical SAM enzyme MoaA accelerates the radical-mediated C-C bond formation by 6-9 orders of magnitude. MoaA catalyzes an unprecedented 3′,8-cyclization of GTP into 3′,8-cyclo-7,8-dihydro-GTP (3′,8-cH2GTP) during the molybdenum cofactor (Moco) biosynthesis. Through a series of EPR, biochemical characterizations, and DFT calculations we found that MoaA catalyzes a shunt pathway in which an on-pathway intermediate, GTP C-3′ radical, abstracts the H-4′ atom from (4′R)-5′-deoxyadenosine (5′-dA) to transiently generate 5′-deoxyadenos-4′-yl radical (5′-dA-C4′) that is subsequently reduced stereospecifically to yield (4′S)-5′-dA. [J. Am. Chem. Soc. 2020, 142, 9314–9326.]

Industry-Trusted Biotechnology Company and CRO

Group of Students Working at the Laboratory

Advance your biomedical research industry with help from CASSIA LLC. Founded in 2004, we are a privately held biotechnology company and clinical research organization (CRO) specializing in green chemo-enzymatic synthesis solutions for preparing stable isotope-labeled (D, C-13, N-15, O-17/18) metabolites, proteins, nucleic acids, and their analogs. 

Leveraging our unique core technology platforms and expertise, we bring responsible, cost-effective, and creative solutions to a variety of basic, translational, and biomedical research applications, such as: 

  • MS 
  • NMR 
  • Assay Development 
  • Metabolic Research 

Our Facility

CASSIA LLC laboratories are located in an eight-building, 150,700 sq. ft. research and development (R&D) business park in the Mira Mesa region of San Diego, CA. We occupy 1580 sq. ft. and are capable of carrying out both basic and applied research in: 

  • Molecular Biology 
  • Biocatalysis 
  • Organic Synthesis 
  • Protein and Nucleic Acid Production 

Contact us today to learn more about our biotechnology company and the products and services we provide. 

Interior of Clean Modern White Medical or Chemical Laboratory Background

Email us to learn more about our services.

Contact Us